Applying the Taguchi Method in Improving the Production of Bacterial Cellulose

doi:10.1166/jbmb.2012.1287

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Please use this identifier to cite or link to this item: http://nhuir.nhu.edu.tw/handle/987654321/26196

Title:	Applying the Taguchi Method in Improving the Production of Bacterial Cellulose
Authors:	林俊宏;Lin, Jun-Hong;Sung, Wen-Pei;Chen, Ting-Yu;Lin, Yi-Jen;Kuo, and Jui-Chih
Contributors:	自然生物科技學系
Keywords:	BACTERIAL CELLULOSE;GLUCONACETOBACTER XYLINUS;PELLICLE THICKNESS;TAGUCHI METHOD;TWO-STAGE CULTIVATION
Date:	2012-12
Issue Date:	2018-10-03 11:43:20 (UTC+8)
Abstract:	Bacterial cellulose (BC) is a type of widely used biopolymer. The production rate of BC can be developed using the Taguchi method and a jar fermentor. By setting and processing the tests of L18(3⁶) orthogonal arrays, the critical factors affecting the BC production are the initial pH and glucose concentration. The optimal factors of a modified YPD medium are the Gluconacetobacter xylinus ATCC 23768, initial pH = 4 5, YPD medium, acetic acid concentration = 1.5% (v/v), glucose concentration = 5% (w/v) and liquid height = 7.2 cm, and can increase 39.0% of the wet film thickness to 3.92 mm. After the two-stage cultivation, the modified YPD-glucose medium can achieve the maximal pellicle thickness of 5.56 mm. With the modified YPD-molasses medium, the BC production rate is higher than that of the glucose medium. A high-cultured volume fraction can obtain a higher thickness and BC production rate. By introducing the two-stage cultivation strategy, the incubation duration can be shortened from 10 days to less than 8.5 days for BC thickness.
Relation:	Journal of Biobased Materials and Bioenergy Vol. 6, no. 6 pp.610-616
Appears in Collections:	[Department of Natural Biotechnology] Periodical Articles

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